LOCUS       40924_41355        7784 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSTH1-GFP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Yamashita M, Hojo M.
  TITLE     Transgenic zebrafish overexpressing HSP70
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Yamashita M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2002) Michiaki Yamashita, National Research 
            Institute of Fisheries Science; 2-12-4 Fukuura, Kanazawa-ku, 
            Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, 
            Tel:81-45-788-7665, Fax:81-45-788-5001)
REFERENCE   3  (bases 1 to 7784)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7784)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2002) Michiaki Yamashita, National Research Institute of 
            Fisheries Science"; volume: " 2-12-4 Fukuura, Kanazawa-ku, Yokohama,
            Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, 
            Tel:81-45-788-7665, Fax"; pages: "81-45-788-5001"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    390..524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      649..1104
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1392..2249
                     /label=AmpR
                     /note="beta-lactamase"
     protein_bind    2354..2387
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2465..3053
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3516..3547
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3555..3571
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3910..4243
                     /note="rainbow trout heat shock cognate protein HSC70
                     promoter"
                     /regulatory_class="promoter"
     CDS             4402..4446
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     regulatory      6465..6470
                     /regulatory_class="polyA_signal_sequence"
     enhancer        6511..6814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6815..7018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             7063..7779
                     /label=EGFP
                     /note="enhanced GFP"
ORIGIN
        1 ggccgctcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
       61 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
      121 gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag tatgatttat
      181 gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag attcacagtc
      241 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata
      301 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      361 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatcct gcattaatga
      541 atcggccaac gcgcggggag aggcggtttg cgtattggct ggcgtaatag cgaagaggcc
      601 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
      661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1021 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1081 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgcca ggtcttggac
     1201 tggtgagaac ggcttgctcg gcagcttcga tgtgtgctgg agggagaata aaggtctaag
     1261 atgtgcgata gagggaagtc gcattgaatt atgtgctgtg tagggatcgc tggtatcaaa
     1321 tatgtgtgcc cacccctggc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2341 ataatctcat gccataactt cgtataatgt atgctatacg aagttatggc atgaccaaaa
     2401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2581 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2821 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     2881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaga gcttgcaatt
     3301 cgcgcgtttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3361 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3421 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3481 ctacgaggcc ctttcactca ttaggcaccc caggctttac actttatagc ttccggctcg
     3541 tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagca tcgtgcaggt
     3601 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3661 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3721 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3781 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3841 catgacctta tgggactttc ctacttggca gtacatctac gggtcgacga gctcactagc
     3901 tcgagatcca ggtcgcagtt gcaaatgaga acttgttctc aactaaccta cctggttaaa
     3961 taaaggttaa ataaaaaaat aaataaataa aaagtgtatc cggtgcgaac gcaacccgac
     4021 gtcttcatgg gaagtgggag ggagcaacag cctgtgtttt tattggtatt ttggaatgtc
     4081 aagttgaatc ctgagctatg attggttgcc ttgccgttta tatagtaatg gtagacttat
     4141 ccttccttct ttctctcaca acacacaagg cgaagaaagg gaggcttcgc cattgttcaa
     4201 ctccgatcaa catcagcatc accttcggtc aaataattta ttcgaattcg ggctgcagga
     4261 attcgatatc aagcttctag agatccctcg acctcgagat ccattgtgct ctaaagacgg
     4321 cagaagctga ctagaacgaa gctctaacct tttcctcaac aaggagatca ccactgactg
     4381 tttatctcaa ccagccatat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg
     4441 gacagcgcca tggcatcagc taagggaata tccattggca tcgatctggg caccacctac
     4501 tcttgtgtcg gggtcttcca gcatggaaaa gtggaaatta tcgccaatga tcaaggcaac
     4561 aggacaactc ccagctacgt ggcctttacc gacacagaga ggctcatcgg agatgctgcc
     4621 aagaaccaag ttgccatgaa ccccaccaac acgatctttg atgccaaacg gctcattgga
     4681 agaaggtttg atgacctggt ggtccagtct gacatgaaac tctggccgtt caaggtgatc
     4741 aacgacaacg gaaagcccaa agtccaggtg gaatataaag gagaaattaa gacattctgt
     4801 cctgaagaga tttcctcgat ggtcctggtt aaaatgagag aaatagctga ggcctttctg
     4861 ggacagaggg tgtcgaatgc ggtcatcaca gtgccagctt actttaatga ttcccaaagg
     4921 caagccacaa aggatgctgg agtgatctcc ggactaaatg ttctccgcat cataaatgag
     4981 ccgacagctg cagccattgc ctacggcctt gataaaggca aaagaggaga gcgcaatgtc
     5041 ctcatctttg atcttggtgg aggcactttt gacgtgtcca tcctgaccat tgaggatggc
     5101 atatttgagg tgaaatccac ggccggggac acacatctcg gcggagagga ttttgacaac
     5161 cgaatggtca gccactttgt ggaggagttt aaaagaaagc acaagaagga catcagccag
     5221 aacaagagag cagtgaggag actgcgcaca gcttgtgaga gagcaaagag gaccttgtcc
     5281 tccagcactc aggcaagcat tgagattgac tcactctttg agggcattga cttttacacc
     5341 tcaatcacaa gggcacgctt cgaggagctc aactcagacc tctttagggg aacactggag
     5401 ccagttgaga aggccctaca agatgctaag ctggataaat ccaagatcca cgaagttgtc
     5461 ctagttggtg gctctacaag aatccccaaa atccaaaagc tcctgcagga cttcttcaat
     5521 ggtagagaac taaacaagag catcaaccct gatgaagcgg tggcctatgg tgcagcagtc
     5581 caggctgcta tcctcatggg tgacacctca gaaaatgtcc aagatttact gctcctggat
     5641 gtggcaccct tgtcgctggg cattgagaca gccggtggcg ttatgacacc tcttatcaag
     5701 cgaaacacca ccatccccac aaagcagact cagatcttct ccacatactc agataaccag
     5761 ccaggagtcc tgattcaggt ctatgaaggt gagagagcca tgacaaagga caacaatctc
     5821 ctggggaagt ttgagctctc tggtcttcct cctgctccca gaggcgtgcc acagatcgaa
     5881 gttacctttg atatcgacgc caacggcatc ctaaatgtgt ctgccgtcga caaaagcacg
     5941 ggcaaagaaa acaaaatcac aatcaccaac gacaagggcc gactcagcaa agatgagatc
     6001 gagaagatgg tgcaggatgc agacaagtac aaggcggagg atgaccagca gagagaaaag
     6061 atcgcggcaa agaattccct ggagtcctac gcttaccaca tgaagagcag cgtcgaagac
     6121 gacgccttga aaggaaaaat cagtgaggag gagaaaaagc tggttattga caaatgcaac
     6181 cagacaattt cctggctgga gaacaaccag ctggcagaga aggaggagta tgaacatcag
     6241 cagaatgagc tggagagggt gtgcaaaccg gtcgtggcaa agttgtatca aggtgcagcg
     6301 gcaggaagct ccagcagtca ggctggagga gccactcagg gtcccactgt cgaggaggtc
     6361 gactaaaact aactgcagca tttgactgct ggataaggat ttctgactta tgtttggttt
     6421 ctgaaaaaaa agcacaatgg atctcgactc tagaggatct cgagaataaa ttacggggtc
     6481 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     6541 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     6601 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     6661 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     6721 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     6781 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     6841 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     6901 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     6961 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     7021 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     7081 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     7141 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     7201 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     7261 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     7321 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     7381 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     7441 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     7501 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     7561 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     7621 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     7681 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     7741 gccgccggga tcactctcgg catggacgag ctgtacaagt aagc
//
